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    Cryo-EM
    Sample Preparation

    Optimized sample and grid preparation is vital to achieving the best possible outcomes in structural biology, by delivering consistently high-quality images and data. 

    Good Sample Preparation for Cryo-EM: What, Why and How?

    Cryo-electron microscopy (cryo-EM) is an invaluable tool in the structural biologist’s toolkit, providing high-resolution information for samples that were previously resistant to other methods such as x-ray crystallography. Researchers are now able to investigate at a single atom level a wider range of proteins, including many important drug targets, to gain a deeper understating of their function within disease related pathways. 

    However, time on high-performance cryo-EM microscopes to collect the necessary data is expensive, and often the availability is restricted. To ensure that you’re getting the most out of your investment, care is needed to optimize your sample concentration, quality and homogeneity, as well choosing the right grid and optimizing your grid preparation protocol. 

    Streamline cryo-EM Sample Preparation

    Optimal cryo-EM grid preparation should maintain sample integrity, minimize damage, reduce contamination, and ensure consistency across experiments. However, it is typically a manual and labor-intensive process, and achieving the optimal grid for data collection can be a challenging task.  

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    chameleon, automate consistency

    chameleon offers unparalleled capabilities in cryo-EM grid preparation, combining next-generation automation, blot-free technology, and high-speed plunging. With intuitive, guided workflows to simplify setup and operation, chameleon removes the major bottleneck in this workflow. 

    chameleon enables users of all experience levels to produce high-quality grids reliably and consistently for a wide range of biological samples, with optimal ice thickness and evenly distributed particles. Its precise, automated grid handling prevents any manual sample loss or damage. The ability to screen grid quality prior to imaging leads to more efficient use of available microscope time which translates through to reducing costs. 

    High-Performance Sample Support

    Support grids play a critical role in the cryo-EM sample preparation process. The nature of your biological sample determines the optimal grid geometry and material, so it is important to choose the right grid that will yield the best results for you, enter Quantifoil.

    Quantifoil, market-leading cryo-electron microscopy support grids

    Quantifoil is the leading provider of cryo-electron microscopy support grids. The growing portfolio features cryo-EM support films with an array of convenient predefined hole sizes, shapes, and arrangements on a full range of grids, to deliver high-performance for all structural biology applications, as well as for the wider electron microscopy community. 

    Correct preparation is essential for obtaining high quality cryo-EM structures every time. By selecting the right support grid and optimizing your grid preparation, you prevent wasted time at the microscope and maintain sample integrity, increasing your chance of success while also improving productivity.  

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    Correct preparation is essential for obtaining high quality cryo-EM structures every time. By selecting the right support grid and optimizing your grid preparation, you prevent wasted time at the microscope and maintain sample integrity, increasing your chance of success while also improving productivity.  

    Contact us today to learn more about our cryo-EM sample preparation solutions and how they can benefit your laboratory operations.