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mosquito

Webinar: Ultra-high throughput experimentation in medicinal chemistry

Join Tim Cernak, Associate Professor at the University of Michigan, and Kamila Koprowska, mosquito® Product Manager at SPT Labtech, as they explore how positive displacement technology and low-volume liquid handling drive scale and throughput in medicinal chemistry.

Webinar

Developing and Embedding In-House Compound Management within Artios

Design, Make, Test and Analyse (DMTA) screening requires the generation of compound assay ready plates (ARP) – a process that can be slow, costly and resource intensive. Negative impact on project cycle times/resource burden. Using dedicated software and automation, we have developed in-house compound management (CM) for DMTA processing and plate generation: Audited and trackable process – increased sample/data integrity. Fully automated workflow for biochemical & cellular assays. Reduced cycle times/cost. Faster data reporting to drive decision making/more effective use of resources.

Poster

BraMosA: Our New Smartcel-2 boosting Serial Dilution Preparation

Enhancing throughput while reducing footprint is a recurring requirement in sample management. With the new Smartcel-2 from Flexible Lab Solutions, we have significantly boosted our capability to prepare assay-ready plates in a serial dilution format. This double-deck automation system features a Bravo from Agilent for high-volume serial dilution plate preparation and a mosquito LV from SPT Labtech for nanoliter dispensing steps. Several peripheral devices, including a peeler, sealer, centrifuge, and plate storage, enable the automation cell to operate around the clock.

Poster

Scaling up plate-based single-cell profiling: introducing automated STORM-seq

Full-length, single-cell RNA-seq (scRNA-seq) allows for more complete transcriptomic profiling compared to 5' or 3' end-counting methods. However, labor-intensive, non-modular workflows with high reagent costs limit scalability. Advancements in laboratory automation, protocol design, and reaction miniaturization have overcome some of these constraints. In collaboration with the Van Andel Institute, we present Single-cell TOtal RNA Miniaturized sequencing (STORM-seq), which pairs Takara Bio’s SMART® technology with SPT Labtech’s mosquito® HV genomics liquid handling platform. This miniaturized, plate-based scRNA-seq approach is scalable, automated, and offers a solution for single-cell transcriptomic profiling (i.e., coding/noncoding transcripts, isoforms, gene fusions, etc.) at reduced cost and increased cell throughput.

Poster

Towards high-throughput low-cost sustainable ecotoxicity testing

Traditional whole animal-based toxicity testing is expensive, time-consuming, and unethical. Furthermore, these tests do not capture the full range of biological impacts. There is growing interest in developing alternative methods (New Approach Methods, NAMs), that are less resource intensive (lower costs, quicker assays, fewer animals), while also providing more data on the mechanism of toxicity. The objective of this work was to optimize and establish a high throughput in vitro transcriptomic-based toxicity testing platform for chemical and environmental risk assessment. The platform couples human Caco-2 and Hep G2 cell lines with QIAGEN’s UPXome ultraplex technology, so that the combined test system can also derive transcriptomic points of departure (tPODs).

Poster

Miniaturization of DNA library preparation for Illumina sequencing using automated positive displacement liquid handling

Advances in next generation sequencing have resulted in huge increases in throughput with associated decreases in costs. As a result, the process of library preparation has become even more of a financial and time constraint to high throughput sequencing core facilities. Automation, in the form of liquid handling robots, has been able to alleviate some of these bottlenecks. This work describes the application of SPT Labtech’s mosquito® HV positive displacement nanoliter liquid handler to successfully implement the preparation of DNA libraries for Illumina sequencing at one tenth the volume of the original manual protocol.

Poster

How to accelerate small molecule lead discovery with automated multichannel pipetting and DEL technology

Establishing an efficient and accurate compound testing workflow is essential to any small molecule screening platform. When utilizing screening methods such as DNA-encoded library (DEL) technology, follow-up hit validation must be performed using traditional biochemical assays. In this webinar, we will review the use of mosquito LV to accelerate early-stage small molecule lead discovery through the lens of Hydroxyacid oxidase 1 (HAO1). In this case study, we will describe our efforts to develop and deploy a robust enzymatic assay towards the development of potent inhibitors identified by DEL technology for disease treatment.

Webinar

mosquito® LV: An Assay’s Best Friend

After a successful DNA-encoded library (DEL) screen, it is essential to have access to a high-throughput method of assaying compounds to validate hits. The ability to create up to sixteen serial dilutions in parallel and subsequently plate those dilutions on a nanoscale can vastly speed up the hit finding process, while improving the accuracy and efficiency of the screen. mosquito® LV by SPT Labtech has greatly improved the way we work with our off-DNA compounds synthesized from DELcore and DELflex collaborations.

Application note

Miniaturisation of DNA library preparation for Illumina sequencing using mosquito® liquid handler

Advances in next generation sequencing have resulted in huge increases in throughput with associated decreases in costs. As a result of these improvements the process of library preparation has become even more of a financial and time constraint to high throughput sequencing core facilities. Automation, in the form of liquid handling robots, has been able to alleviate some of these bottlenecks. This study describes the application of one of these systems, the mosquito HV system, to successfully implement the preparation of DNA libraries for Illumina sequencing in one tenth the volume of the original manual protocol.

Application note

Expanding High Throughput Nanolitre Solution-Phase Crystallisation for Polymorph Screening

Polymorphism is a solid-state phenomenon where a chemical species may adopt different conformational or packing arrangements, therefore allowing for the formation of more than one distinct crystal structure. Crystal polymorphs can exhibit varying physical and chemical properties. These properties such as solubility, hardness, colour, and melting point can all be important in the design of a compound in the crystal engineering and pharmaceutical industries.1, 2 Typically, conducting polymorph screens requires a combination of classical crystallisation techniques which demand both large quantities of material and time to complete. Herein, we describe how encapsulated nanodroplet crystallisation (ENaCt) can be applied for low-quantity rapid polymorph screening using highly polymorphic 5-methyl-2-[(2-nitrophenyl)amino]-3thiophenecarbonitrile (ROY) and nicotinamide as test compounds. ENaCt polymorph screening resulted in the growth, and analysis, of single crystals of a new polymorph of ROY ‘O22’. Crystal forms of both ROY and nicotinamide previously thought to be inaccessible to solution-phase crystallisation were also successfully grown, highlighting the capabilities of ENaCt for screening a diverse polymorphic landscape.

Poster

SLAS 2023 Interview - Chris Waddling - dynamic duo

Discover how genomics researchers can miniaturize NGS methods to increase throughput and lower costs using the combination of the mosquito® and dragonfly® automated liquid handlers from SPT Labtech in this interview with Field Application Scientist, Chris Waddling.

Video

encapsulated nanodroplet crystallisation (ENaCt) for small organic molecules: polymorph discovery

Crystallisation is of vital importance to the drug development pipeline of the pharmaceutical industry due to the existence of multiple crystal forms (e.g. cocrystals, solvates and polymorphs) of an active pharmaceutical ingredient (API). The physical properties of the crystal form of an API are important in manufacturing, API stability and can have significant impact on the rate of API uptake from the solid form (e.g. from a tablet), ultimately influencing both its formulation chemistry and the pharmacokinetics of the API.

Application note

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