Paper
Miniaturization of Smart-seq2 for Single-Cell and Single-Nucleus RNA Sequencing
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Increase your productivity and reduce the cost per sample by miniaturizing your NGS method with proven workflow solutions for over 40 NGS kits, including industry standards such as Illumina Nextera XT, Illumina DNA Prep, NEBNext Ultra II, plus many more.
Volume miniaturization with our automated liquid handlers – mosquito® and dragonfly® – solves throughput and cost challenges, enabling genomics researchers to process more samples and make savings on reagent use.
Our 'dynamic duo' combines mosquito's highly accurate positive displacement pipetting with dragonfly's non-contact, nanoliter-to-milliliter dispensing. mosquito gives you full visibility with the ability to aspirate, dispense, mix, and perform on-plate serial dilutions, while dragonfly delivers accurate nanoliter dispensing irrespective of liquid viscosity or environmental conditions. Increase the productivity of your workflow with successful miniaturization solutions for a growing list of NGS methods and qPCR/PCR setups.
See the links to resources and customer success stories in the methods table below: over 40 NGS methods successfully miniaturized.
Introducing our most compact and accessible member of the mosquito family. mosquito Gen3 offers highly accurate and precise multi-channel pipetting from 500 nL to 5 µL, allowing genomics researchers to benefit from miniaturization across a range of applications, irrespective of liquid class. Its rapid plate-to-plate pipetting motion and tip exchange maximize speed and efficiency.
Explore mosquito Gen3For those looking for higher throughput and greater walkaway capacity, mosquito HV genomics delivers the same highly accurate and precise multi-channel pipetting but with two additional deck positions. Run full miniaturized workflows with less hands-on time, and have confidence handling even the most viscous and volatile liquids from 500 nL to 5 µL.
Explore mosquito HV genomicsIf you're looking to work with even smaller volumes, then mosquito LV genomics is for you. This instrument delivers exceptionally accurate and precise multi-channel pipetting from 25 nL to 1.2 μL, enabling you to extend the reach of precious samples and reagents even further.
Explore mosquito LV genomicsdragonfly discovery enables innovative, low volume reagent dispensing across a broad range of applications including genomics, assay development, and 3D cell cultures. Highly repeatable and accurate low volume dispensing, rapid large volume dispensing, and ultra-low dead volumes allow researchers to minimize reagent costs, save time, and standardize assays for superior quality data.
Explore dragonfly discovery*Scroll left and right to view more information
Application Area
Kit Name
Instrument Model
Standard Volume [µL]
Miniaturized Volume [µL]
N-Fold
Citations (hover for resource name)
SINGLE CELL/LOW INPUT CDNA SYNTHESIS (full transcriptome)
SMART-Seq2
25
2.5
Up to 10x
SMART-Seq V3
7
3.5
Up to 2x
Takara SMART-Seq v4
50
12.5
4x
Takara SMART-Seq Single Cell
50
7
~7x
Takara SMART-Seq Stranded
100
25
4x
SINGLE CELL/LOW INPUT CDNA SYNTHESIS(3’ counting)
CEL-seq2
12
2.6
~5x
Ribo-seq
5.79
5.79
-
SINGLE CELL/LOW INPUT WHOLE GENOME AMPLIFICATION
Qiagen REPLI-g Single Cell
50
1
50x
DigiPico
6.2
6.2
-
DNA SEQUENCING
Illumina Nextera XT
50
2
Up to 25x
Illumina DNA Prep
50
5
Up to 10x
Invitrogen Collibri ES DNA
50
5
10x
NEBNext Ultra II FS DNA
50
5
10x
KAPA HyperPlus
100
10
10x
modified Twist Library Preparation EF Kit
48.75
4.9
Up to 10x
RNA SEQUENCING
NEBNext Ultra II Directional RNA
50
5
10x
NEBNext Multiplex Small RNA
100
20
5x
Takara SMARTer Stranded Total RNA-Seq
100
12.5
8x
AMPLICON SEQUENCING
COVID-seq
50
25
2x
Illumina 16S Library Prep
50
5
10x
Ion AmpliSeq Library Kit 2.0
30
2.4
Up to 12.5x
SAMPLE AND LIBRARY BEAD CLEAN-UP, QUANTIFICATION
Beckman AMPure XP, SPRIselect
variable
>10x
Up to 10x
Biotium AccuClear
variable
variable
Up to 15x
Invitrogen Collibri Library Quant
20
2.5
Up to 8x
Promega QuantiFluor
variable
variable
Up to 30x
Quant-iT PicoGreen
variable
variable
Up to 150x
Roche KAPA Library Quant
20
2.5
Up to 8x
qPCR/PCR SETUP
gene expression profiling (Fluidigm Biomark HD)
23
-
-
other
variable
variable
Up to 50x
OTHER
Olink Explore
19.8
-
-
HiFiViral SARS-CoV-2
-
-
-
The liquid handling software that controls the mosquito family of instruments gives users an intuitive experience tailored to their application area.
Developed in collaboration with scientists, the latest mosquito software facilitates miniaturization in novel application areas. Packed with powerful features, the software includes an experiment designer and helpful wizards to create and manage your protocols.
Watch Dr. Eric Schordan, Chief Scientific Officer at Firalis Molecular Precision, and Dr. Kamila Koprowska, Senior Business Development Scientist at SPT Labtech, as they explore the transformation of large-scale proteomic data generation using the Olink® Explore platform integrated with positive displacement liquid handling technology.