Dynamic duo for miniaturizing NGS methods

Increase your productivity and reduce the cost per sample by miniaturizing your NGS method with proven workflow solutions for over 40 NGS kits, including industry standards such as Illumina Nextera XT, Illumina DNA Prep, NEBNext Ultra II, plus many more.

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Illumina, PacBio, Nanopore … boost the productivity of your NGS workflows

Volume miniaturization with our automated liquid handlers – mosquito® and dragonfly® – solves throughput and cost challenges, enabling genomics researchers to process more samples and make savings on reagent use.

Our 'dynamic duo' combines mosquito's highly accurate positive displacement pipetting with dragonfly's non-contact, nanoliter-to-milliliter dispensing. mosquito gives you full visibility with the ability to aspirate, dispense, mix, and perform on-plate serial dilutions, while dragonfly delivers accurate nanoliter dispensing irrespective of liquid viscosity or environmental conditions. Increase the productivity of your workflow with successful miniaturization solutions for a growing list of NGS methods and qPCR/PCR setups.

See the links to resources and customer success stories in the methods table below: over 40 NGS methods successfully miniaturized.

 

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mosquito® Gen3

Introducing our most compact and accessible member of the mosquito family. mosquito Gen3 offers highly accurate and precise multi-channel pipetting from 500 nL to 5 µL, allowing genomics researchers to benefit from miniaturization across a range of applications, irrespective of liquid class. Its rapid plate-to-plate pipetting motion and tip exchange maximize speed and efficiency.

Explore mosquito Gen3

mosquito® HV genomics

For those looking for higher throughput and greater walkaway capacity, mosquito HV genomics delivers the same highly accurate and precise multi-channel pipetting but with two additional deck positions. Run full miniaturized workflows with less hands-on time, and have confidence handling even the most viscous and volatile liquids from 500 nL to 5 µL.

Explore mosquito HV genomics
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mosquito® LV genomics

If you're looking to work with even smaller volumes, then mosquito LV genomics is for you. This instrument delivers exceptionally accurate and precise multi-channel pipetting from 25 nL to 1.2 μL, enabling you to extend the reach of precious samples and reagents even further.

Explore mosquito LV genomics

dragonfly® discovery

dragonfly discovery enables innovative, low volume reagent dispensing across a broad range of applications including genomics​, assay development, and 3D cell cultures. Highly repeatable and accurate low volume dispensing, rapid large volume dispensing, and ultra-low dead volumes allow researchers to minimize reagent costs, save time, and standardize assays for superior quality data.

Explore dragonfly discovery
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Workflow examples

Click on the workflow to expand.

Over 40 NGS methods miniaturized​

*Scroll left and right to view more information

Application Area

Kit Name

Instrument Model

Standard Volume [µL]​

Miniaturized Volume [µL]​

N-Fold

Citations (hover for resource name)

SINGLE CELL/LOW INPUT CDNA SYNTHESIS ​(full transcriptome)

SMART-Seq V3

mosquito Gen3 mosquito HV genomics

7

3.5

Up to 2x

Takara SMART-Seq v4

mosquito Gen3 mosquito HV genomics

50

12.5

4x

Takara SMART-Seq Stranded​

mosquito Gen3 mosquito HV genomics

100

25

4x

DNA SEQUENCING

Illumina DNA Prep

mosquito Gen3 mosquito HV genomics dragonfly discovery

50

5

Up to 10x

Invitrogen Collibri ES DNA

mosquito Gen3 mosquito HV genomics

50

5

10x

NEBNext Ultra II FS DNA

mosquito Gen3 mosquito HV genomics

50

5

10x

KAPA HyperPlus

mosquito Gen3 mosquito HV genomics

100

10

10x

modified Twist Library Preparation EF Kit

mosquito Gen3 mosquito HV genomics

48.75

4.9

Up to 10x

SAMPLE AND LIBRARY BEAD CLEAN-UP, QUANTIFICATION

Biotium AccuClear

mosquito Gen3 mosquito HV genomics mosquito LV genomics

variable

variable

Up to 15x

Invitrogen Collibri Library Quant

mosquito Gen3 mosquito HV genomics

20

2.5

Up to 8x

Promega QuantiFluor

mosquito Gen3 mosquito HV genomics mosquito LV genomics

variable

variable

Up to 30x

Quant-iT PicoGreen

mosquito Gen3 mosquito HV genomics mosquito LV genomics

variable

variable

Up to 150x

Roche KAPA Library Quant

mosquito Gen3 mosquito HV genomics

20

2.5

Up to 8x

OTHER

HiFiViral SARS-CoV-2

mosquito LV genomics dragonfly discovery

-

-

-

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Software that's intuitive by design

The liquid handling software that controls the mosquito family of instruments gives users an intuitive experience tailored to their application area.

Developed in collaboration with scientists, the latest mosquito software facilitates miniaturization in novel application areas. Packed with powerful features, the software includes an experiment designer and helpful wizards to create and manage your protocols.

Learn more about the mosquito software

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Watch our recent webinar

Watch Dr. Eric Schordan, Chief Scientific Officer at Firalis Molecular Precision, and Dr. Kamila Koprowska, Senior Business Development Scientist at SPT Labtech, as they explore the transformation of large-scale proteomic data generation using the Olink® Explore platform integrated with positive displacement liquid handling technology.

Watch the webinar recording