Advanced operations - Initial VolumeCheck set up conditions

  • Computer is ON and VolumeCheck is ON.
  • Allow the VolumeCheck instrument to “warm-up” for 15 minutes.
    VC icon
  • Double-click on the VolumeCheck icon on the desktop to start the VolumeCheck software.
  • The Main Processing Screen is displayed.

Setting up Output files
The VolumeCheck generates an Output file for each well plate. If needed, setup Output file fields
and directory.

  • Select the Settings Menu, then Output Options, then select appropriate output fields and
    destination folder.

Setting scanning options and error handling

  • Typically the VolumeCheck is set to take 3 scans of each well to allow calculation of the
    standard deviation. In addition, error handling can be set to re-scan if data is out of range or
    standard deviation is too high.
  • Select Settings Menu, then Ultrasonic Scanning Options, then select appropriate settings.
    Example settings are: scans per sample = 3, wait time = 10, rescan min mm = 10, rescan.
  • See scanning options for more details about system settings.

Select a Calibration File

  • Click on LOAD TABLE button in the upper right corner of main processing screen.
  • (If a calibration table is not available continue to the next step. Sensor-to-sample distance
    data will be collected.)

Note: After scanning a rack you can click on the LOAD TABLE button to switch between calibration tables.

Load well plate or tube rack into VolumeCheck to scan

  • Place the well plate or test tube rack onto the VolumeCheck input tray.

Note: Tube racks or well plates are oriented with tube (or well position) A01 towards the rear left of the VolumeCheck input tray.

  • Press the Start button to initiate the VolumeCheck scan of the plate.

Note: for information on how to create a calibration file, please see the Plate Data Creator section.

Save output file

  • Once the plate scan is complete, the input tray opens.
  • The software prompts you to enter the plate or rack ID number. (Type RACK ID number to
    name the output file or use a handheld barcode scanner to scan the ID barcode to name the
    output file).
  • Repeat the above steps for each well plate.

Save Project

  • Once all settings are confirmed, click OK. Settings can be saved as a project file that can be
    used in the future. Select File Menu and then Save Project.