Webinar
Elucidating cost-effective miniaturized mRNA-seq library construction and implications for the study of circadian rhythms

Dr. Esser’s group at the University of Florida previously showed that cellular circadian clocks direct a daily transcriptional program that supports homeostasis and resilience. To further learn the age-associated changes in circadian functions, they profiled the circadian transcriptome in different tissues including skeletal muscle, the heart, and others.

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About this webinar

To speed up the library construction and potentially save reagents cost, we manually tested ½x, ¼x, 1/5x and 1/10x reduced reaction volumes for RNA-seq library construction using NEB Ultra Directional RNA-seq kit. The results showed that 1/5x gave us reliable results. Thus, by leveraging the mosquito® and dragonfly® discovery automated liquid handling systems for mRNA-seq library construction with 1/5x reduced reaction, reagent costs were reduced five-fold via miniaturization whilst maximizing the reliability of an automated workflow.

Key learning objectives

  • Explore the first-hand experience of automation-enabled miniaturization of mRNA-seq
  • Learn about the first-hand experience of the utility of mosquito and dragonfly discovery for NGS in a shared core facility
  • Understand the fundamental mechanism of molecular clocks and their impact on physiology, using skeletal muscle as a tissue focus
  • Discover the application of sequencing tools to investigate the influence of circadian rhythms, particularly with aging

 

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