Webinar
Driving Throughput in tRNA Sequencing Library Generation With Automated Liquid Handling

About this webinar

Transfer RNAs (tRNAs) are critical adaptor molecules that translate genetic information into functional protein sequences, dictating the fidelity of translation processes in the cell. High-resolution sequencing-based methods for tRNA sequencing have provided transformative insights into the physiological impact of tRNA expression, modification, and aminoacylation (charging) levels on cellular stress and disease conditions. Additionally, large-scale genome engineering efforts have employed tRNA sequencing to recapitulate the translational state of engineered cells. However, tRNA sequencing has not been widely adopted due to the time-consuming, arduous, and specialized nature of library generation workflows.

In this webinar, Russel Vincent of the Church Lab and Biopolymers Genomics Core Facility (BPF) at Harvard Medical School will describe how he and colleagues streamlined library generation for tRNA sequencing using SPT Labtech’s firefly all-in-one automated liquid handling platform. This method delivers significant time savings in the lab by reducing a previously multi-day workflow to a single day, while improving throughput and reducing experimental variation.

The speakers will present:

• An introduction to automated tRNA Sequencing: Hear first-hand experience of automation-enabled tRNA sequencing technology.
• How the versatility of the firefly liquid handling platform enables the development of reliable and modular library preparation protocols for NGS.
• Sequencing applications, such as genome engineering, genetic code expansion, and investigating cellular stress.

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