Automated Library Preparation using Watchmaker DNA Library Prep Kit with Fragmentation on firefly®

With the rapid growth of Next Generation Sequencing (NGS), having a robust automation-friendly library preparation solution is a minimum requirement for the handling of precious samples across many applications. Automating the library prep process has the benefits of not only increasing throughput, but also minimizing performance variability and can even help to eliminate errors in sample-tracking. However, the transition from manual workflows to automation can often be daunting and time-consuming. This application note describes one lab’s process, TGen North, to evaluate several library prep solutions and automation systems with the goal of transitioning from their current manual library prep to an automated workflow using SPT Labtech’s firefly® along with the Watchmaker DNA Library Prep Kit with Fragmentation.